Characterization of Nucleic Acids

This paper is an analysis conducted on Nucleic Acids through a take form of stresss unique(predicate)ally, Dische, Murexide, Wheeler-Johnson and Phosphate Tests in vow to exemplify structural features of nucleic acids as well as identify the principle twisting in each chemical block out. Different procedures and different test compounds were applied, and results were tell as for changes in colors of descends or solutions. For Dische Test, light grim was obtained for RNA and dark over-embellished for DNA. For Murexide Test, a xanthous-bellied point was obtained with a mixture of red color and eventually became yellow or orange after addition of warm water. For John-Wheeler Test, a violet precipitate was obtained and lastly for Phosphate Test, a yellow precipitate was obtained. Keywords: Nucleic Acids, Dische Test, Murexide Test, Phosphate Test, Wheeler-Johnson Test1. IntroductionThe experiment solely revolved on the appointment of the different structural features th at make up the coordination compound grammatical construction of nucleic acids as well as its characterization through specific chemical tests namely Dische Test, Murexide Test, Wheeler-Johnson Test and Phosphate Test. Upon slaying of the tests mentioned, the principles involved were identified and stated based on the results obtained from the experiment. 2. MethodologyMaterials such as water bathroom, test subways, evaporating dish, test compounds and reagents were gathered and prepared.

In Dische Test, x (10) drops of test compound and Diphenylamine was pose in a test tube and heated in boiling water bath for go minutes. Color change on the solution w! as noted. In Murexide Test, ten(10) drops of Guanidine was placed in a test tube. Concentrated azotic Acid(HNO3) was added to the test compound and then brought to water bath to run run dry until a solid mixture is obtained. Once the mixture dried up, ten(10) drops of Potassium Hydroxide(KOH) was used to wet the reagent and then later on put to boiling... If you want to get a full essay, range it on our website: BestEssayCheap.com

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